ExPlain™ is a unique data analysis system that combines promoter and pathway analysis tools. Using the power of TRANSFAC®’s transcription factor binding site derived positional weight matrices, ExPlain™ enables you to identify transcription factors affecting gene expression in your microarray and RNA-Seq experiments, as well as predict how they, in combination, can induce observed gene expression patterns. With PROTEOME™’s pathway data, you can take your analysis further and gain insight into the key upstream signaling regulators influencing the activity of these transcription factors.
Benefits
- Perform high throughput prediction of transcription factor binding sites
- Model how transcription factors act together to affect gene expression patterns observed in microarray and RNA-Seq experiments
- Understand the cause, not just the effect, of differential expression in response to drug treatment, disease state, environmental conditions, and more
- Identify potential therapeutic targets or biomarkers acting upstream of your differentially expressed gene set
Key Features
- Advanced data management options for processing and filtering your microarray, ChIP-Seq and RNA-Seq data
- Specialized algorithms for optimized transcription factor binding site prediction
- Positional weight matrix algorithms for superior accuracy
- Combined positional weight matrix and pattern matching algorithms for superior sensitivity
- Genetic algorithms for generating complex promoter models
- Discovery algorithms for identifying novel DNA motifs
- Specialized algorithms and tools for advanced network and pathway analysis
- Fully interlinked with TRANSFAC® and PROTEOME™ reports
Access Options
Online
An online subscription provides access to the ExPlain web interface. Online subscriptions are available at the concurrent user, academic / non-profit lab, and institution-wide level. The concurrent user and academic / non-profit lab subscription levels require IP plus username/password authentication.
Resources
Support Documents
- Application Note: ExPlain™ Analysis of TAL1 ChIP-seq Intervals (.pdf)
- Getting started guide (.pdf)
- Match and F-Match FAQs (.pdf)
- Key Node Analysis FAQs (.pdf)
Training
Release 2012.4 – Available on-demand
>> Watch it on YouTube
Video Tutorials
Data Input Types
Upload and analyze various data types.
>> Watch video
MATCH to Keynode Analysis
Promoter analysis allows you to find putative transcription factor binding sites in the promoters of a gene set or user-loaded sequences.
>> Watch video
Functional Analysis Module
Allows you to investigate the biological properties of any gene list.
>> Watch video
Archived Webinars
Upstream Analysis of Gene Expression Data
Analysis of TAL1 ChIP-seq Intervals